homoGel®
homoGel® –
Revolutionizing Biomedical Research with Human-Derived ECM
homoGel® is an innovative, native human-derived extracellular matrix (ECM) that redefines standards in biomedical research and tissue engineering. Engineered to surpass the limitations of conventional animal-derived scaffolds, such as those from mice and pigs, homoGel® offers superior biological relevance. Unlike synthetic hydrogels, which are inert and lack interaction with human cells, and plant-based hydrogels, which are predominantly composed of polysaccharides like cellulose, homoGel® provides a highly interactive and authentic environment for human cell studies. Its unmatched consistency, scientific accuracy, and ethical alignment make it the optimal choice for researchers focused on creating precise, human-specific tissue models for advanced applications.
Key Features and Benefits
- Human Specificity:
homoGel® is derived from native human tissues, providing the precise biochemical and structural support necessary for human cells. This ensures that tissue models closely mimic the natural human environment, leading to more reliable and predictive outcomes in drug testing, disease modeling, and other critical research areas. - Consistency:
homoGel® is produced under strict quality control measures, ensuring minimal batch-to-batch variability. This consistency is crucial for reproducibility, a cornerstone of scientific research, enabling more reliable and validated results across different studies and laboratories. - User-Friendly:
homoGel® is transparent, optimally tuned for stiffness, and designed for ease of use, making it simple to handle and pipette — even for individuals with limited experience in cell culture laboratories. - Ethical Advancement:
homoGel® is an animal free product and thus reduces the reliance on animal-derived products, aligning with the growing demand for humane and sustainable research practices. This ethical approach not only addresses concerns about animal welfare but also enhances the scientific validity of research by focusing on human-relevant models.
What are typical applications?
Typical applications for homoGel® span a wide range of research fields, including organoid culture, angiogenesis regulation, cancer research, regenerative medicine, and tissue engineering and organ reconstruction.
Organoid Culture:
homoGel® supports growth of organoids that more accurately replicate human organ structures and functions, leading to better disease models and more effective drug testing platforms.
Cancer Research:
homoGel® provides a humanspecific environment that is critical for understanding tumor-ECM interactions, aiding in the development of new cancer treatments.
Angiogenesis and vascularization:
homoGel® is not merely a passive scaffold; it actively regulates and guides angiogenesis by storing angiogenic factors & influencing the process through mechanical cues based on its physical stiffness.
Regenerative Medicine:
homoGel® is the scaffold for MSC and iPSC growth, serving as a cornerstone in preclinical medical testing and the development of therapeutic models for regenerative diseases and tissue repair.
Tissue Engineering & Organ Reconstruction: homoGel® is designed to support a robust platform for in vitro organ and tissue reconstruction, as well as advancements in bioprinting development.
homoGel® – Revolutionizing Biomedical Research with Human-Derived ECM
Product Description
homoGel® is a native Human Extra Cellular Matrix derived from Human Tissues.
Storage/Stability
- Store the product at –20°C for long-term storage.
- It is recommended, to aliquot the homoGel® after the first thawing and store it at -20°C.
- Avoid multiple freeze/thaw cycles.
- homoGel® may be stored at 2-8°C for up to 48 hours.
Handling
- Thaw homoGel® overnight at 2-8°C before use.
Note: After thawing, the gel may be slightly turbid, but this does not affect its quality. - Mix homoGel® solution by slowly pipetting up and down. Be careful not to introduce air bubbles.
1. How to use the homoGel® for coating the growth surface
- Dilute the homoGel® 1:10 with pre-chilled (4°C) DPBS.
Note: Determine the optimal coating concentration for your application. If needed, adjust the dilution. - Pipet 150μL diluted homoGel® per cm2 onto the growth surface.
- Incubate the coated plate for 10 minutes at room temperature.
- Aspirate the supernatant and dry the growth surface with lid open for 30 minutes under aseptic
conditions. - The coated vessel are ready for use. You can also seal it airtight and store it at 2-8°C for 5 days at
humid conditions.
2. How to use the homoGel® to form Spheroides
- Dilute the homoGel® 1:50 with pre-chilled (4°C) culture medium (2% final concentration).
Note: Determine the optimal concentration for your application. If needed, adjust the dilution. - Mix the medium gently and heat it to 37°C.
- Harvest cells from culture and dilute them into the pre-warmed media.
- Generally, cells are diluted 1 x 104 – 5 x 104 cells/mL, depending on cell line and assay conditions.
- Add 1mL of the cell suspension to each well of a 24-well Ultra-Low-Attachment Plate.
- Incubate the plate at 37°C in a humidified atmosphere and 5% CO2.
- Monitor growth by brightfield microscopy.
3. How to use the homoGel® to form Organoids
- Warm culture vessels in a 37°C incubator for at least 60 minutes.
- Harvest cells from culture and centrifuge the required number of cells.
Generally, 500 – 1.000 cells/μL ECM are seeded.
Note: Determine the optimal concentration for your application. If needed, adjust the cell concentration. - Carefully aspirate the supernatant from the centrifuged cells, without disturbing the cell pellet.
- Add the required amount of homoGel® and resuspend the cell pellet in the gel by carefully
pipetting it up and down several times. Be careful not to introduce air bubbles. - 20μL domes are seedet to the pre-warmed culture vessels. When seeding is complete, cover plate
with its lid and carefully invert it. - Incubate the inverted plate for 2 hours at 37°C in a cell culture incubator.
- After gelling, return the plate in the normal orientation. Carefully add pre-warmed (37°C) culture
media to the wells without disturbing the domes. - Return plate to the cell culture incubator. Monitor growth by brightfield microscopy.
Quality Control
homoGel® is subject to comprehensive quality tests, summarized in the
accompanying certificate of analysis.
Donors have been tested and found to be negative for anti-HIV; anti-HCV and HBsAg.
Warning note
Concerning use of biological material: BIOMEX homoGel® are of human origin, and no known test procedures can ensure the total absence of infectious agents. All products should therefore be handled following safety precautions as if they were infectious.
For research use only
Not intended for any human or animal diagnostic or therapeutic use.
Our Experts for Diagnostics
At BIOMEX, we believe that working with our customers is the best way to improve project success and reduce costs and development time.
