homoGel®
homoGel® –
Revolutionizing Biomedical Research with Human-Derived ECM
homoGel®is an innovative, native, human-derived extracellular matrix (ECM) that redefines standards in biomedical research and tissue engineering. Engineered to surpass the limitations of conventional animal-derived scaffolds, homoGel® offers superior biological relevance. Unlike synthetic hydrogels, which do not fully recapitulate interactions with human cells, and plant-based hydrogels, which are predominantly composed of polysaccharides like cellulose, homoGel® provides a highly interactive and authentic environment for human cell studies. Its unmatched consistency, scientific accuracy, and ethical alignment make it the optimal choice for researchers focused on creating precise, human-specific tissue models for advanced applications.
Key Features and Benefits
- Human Specificity:
Derived from native human tissues, homoGel® provides the exact biochemical and structural environment needed for human cells. This high level of biological relevance ensures that tissue models closely replicate the natural human tissue specific microenvironment, enhancing predictability and reliability in drug testing, disease modeling, and regenerative medicine. - Consistency:
Produced under stringent quality control protocols, homoGel® ensures minimal batch-to-batch variability. This consistency is essential for reproducibility, which is a fundamental aspect of scientific research, providing validated results across different studies, laboratories, and applications. - User-Friendly:
With its transparent structure, optimized stiffness, and easy handling properties, homoGel® simplifies experimental procedures. It is designed for straightforward use, making it ideal for researchers at all levels of expertise, from seasoned professionals to those new to cell culture techniques. - Ethical Advancement:
As an animal-free product, homoGel® supports humane and sustainable research practices by eliminating the need for animal-derived materials. This ethical approach aligns with the global shift towards more humane research standards, enhancing the credibility and relevance of human-based scientific studies.
What are typical applications?
Typical applications for homoGel® span a wide range of research fields, including organoid culture, angiogenesis regulation, cancer research, regenerative medicine, tissue engineering and organ reconstruction.
Organoid Culture:
By providing a human-specific ECM environment, homoGel® promotes the growth, differentiation, and structural integrity of organoids. This results in more accurate disease models and enhanced platforms for drug discovery, toxicity testing, and personalized medicine.
Cancer Research:
With its biologically relevant matrix, homoGel® serves as a critical tool for investigating tumor-ECM interactions and transformations. It enables the development of realistic in vitro cancer models, aiding researchers in understanding tumor growth, metastasis, and the efficacy of new therapeutics.
Angiogenesis and vascularization:
Unlike conventional matrices, homoGel® actively regulates and guides angiogenesis through its unique composition. By offering natural binding sites for endothelial cells and providing mechanical cues, homoGel® supports the formation of complex vascular networks essential for advanced tissue models.
Regenerative Medicine:
homoGel® is the scaffold for MSC and iPSC growth, serving as a cornerstone in preclinical medical testing and the development of therapeutic models for regenerative medicine and tissue repair.
Tissue Engineering & Organ Reconstruction: Designed for in vitro organ and tissue reconstruction, homoGel® provides a reliable matrix for stem cell bioprinting and structural tissue engineering. Its exceptional compatibility and support for cellular attachment and growth make it a powerful tool for developing next-generation biomedical solutions.
homoGel® – Revolutionizing Biomedical Research with Human-Derived ECM
Product Description
homoGel® is a Human Extra Cellular Matrix by BIOMEX.
Storage/Stability
- Store the product at -20 °C for long-term preservation.
- After the first thaw, aliquot homoGel® and store it at -20 °C.
- Avoid repeated freeze/thaw cycles.
- homoGel® can be stored at 2-8 °C for up to 48 hours.
Handling Instructions
- Thaw homoGel® overnight at 2-8 °C before use.
Note: The gel may appear slightly turbid after thawing, but this does not affect its quality - Gently mix the homoGel® solution by slowly pipetting up and down to avoid the formation of air bubbles.
1. How to use homoGel® to coat the growth surface
- Dilute homoGel® 1:10 to 1:100 with pre-chilled (4 °C) DPBS.
Note: Determine the optimal coating concentration for your specific application and adjust dilution if necessary. - Dispense 150 µL of the diluted homoGel® per cm² onto the growth surface.
- Incubate the coated plate at room temperature for 15 to 20 minutes.
- Aspirate the supernatant.
- The coated vessel is now ready for use. Alternatively, you can seal it airtight and store it at 2-8°C in humid conditions for up to 2 days.
2. How to use homoGel® to form Spheroides
- Dilute the homoGel® 1:50 with pre-chilled (4°C) culture medium (2% final concentration).
Note: Determine the optimal concentration for your specific application, and adjust the dilution if necessary. - Harvest cells from culture and resuspend them in the pre-warmed medium.
Note: Typically, cells are diluted to a concentration of 1 x 10⁴ – 5 x 10⁴ cells/mL, depending on the cell line and assay requirements. - Add 1 mL of the cell suspension to each well of a 24-well Ultra-Low-Attachment plate.
- Incubate the plate at 37 °C in a humidified atmosphere with 5% CO₂.
- Monitor spheroid growth using brightfield microscopy.
3. How to use homoGel® to form Organoids
- Warm culture vessels in a 37 °C incubator for at least 60 minutes or longer, as needed.
- Harvest cells from culture and centrifuge the suspension, to collect the required number of cells.
Note: Typically, 500 – 1,000 cells/µL ECM are seeded. Adjust the cell concentration if necessary for your application. - Carefully aspirate the supernatant from the centrifuged cells without disturbing the cell pellet.
- Add the required amount of homoGel® and gently resuspend the cell pellet by pipetting up and down several times. Avoid introducing air bubbles.
- Seed 20 µL domes onto the pre-warmed culture vessels. Once seeding is complete, cover the plate with its lid and carefully invert it.
- Incubate the inverted plate for 2 hours at 37 °C in a cell culture incubator to allow gelling.
- After gelling, return the plate to its normal orientation. Carefully add pre-warmed (37°C) culture media to the wells without disturbing the domes.
- Return the plate to the cell culture incubator and monitor organoid growth using brightfield microscopy.
Quality Control
All BIOMEX homoGels® are subject to comprehensive quality testing, as detailed in the accompanying Certificate of Analysis.
All donors have been tested and confirmed negative for Anti-HIV, Anti-HCV, and HBsAg.
Warning note
Regarding the Use of Biological Material:
BIOMEX homoGel® is of human origin, and no current test procedures can guarantee the complete absence of infectious agents. Therefore, all products should be handled with safety precautions as if they were potentially infectious.
For research use only
Not intended for diagnostic or therapeutic use in humans or animals.
DS -28.11.24/ v2
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